Enzymes and Enzyme Systems per se
4385120 THERMOSTABLE GLYCEROKINASES PROCESS
Anthon Atkinson, Michael Comer. Salisbury, United Kingdom, assigned to the Secretary of State for Defense in Her Britannic Majesty’s Government of the United Kingdom of Great Britain and Northern Ireland A thermostable glycerokinase enzyme useful in the detection and estimation of glycerol derivatives has a half-life in excess of I hour at 55 degrees C. at a protein concentration of less than 2 MG,IML and a pH of 7.8 +:-0.5 in the absence of any substrate. The enzyme is produced by culturing at least one micro-organism which is capable of growth at a temperature of at least 50 degrees C. in a culture medium in which it will produce said enzyme, disrupting the resulting cells of the micro-organism to release the enzyme and separating the enzyme from the cell debris. The medium normally contains at least 0.1% glycerol but certain strains of micro-organism have been found to be capable of producing thermostable glycerokinase enzyme even in the absence of glycerol. The micro-organism is preferably a bacillus organism, especially of the stearothermophilus species.
A microorganism strain B-078 I belonging to the genus pseudomonas isolated from a soil sample from an onion field in Japan. produces a novel enzyme nucleoside oxidase having substrate specificity on various nucleosides and enzyme action to catalyze enzymatic reactions involving various nucleosides. The novel nucleoside oxidase is produced by culturing a nucleoside-oxidaseproducing microorganism belonging to the genus pseudomonas in a nutrient medium containing assimilable carbon and nitrogen and inorganic salt, and isolating the thusformed nucleoside oxidase from the cultured cells. Various nucleoside-5’-carboxylic acids can be produced. by incubating the novel nucleoside oxidase with a nucleoside having a 5’-hydroxymethyl group. in an aqueous medium under aerobic conditions. and isolating the thus-formed nucleoside-5’carboxylic acid from the incubation medium. Assay methods for nucleosides in liquid samples are provided, which comprise incubating the sample with nucleoside oxidase, thereby consuming oxygen and generating hydrogen peroxide and nucleoside-5’carboxylic acid by acting on nucleoside. and quantitatively determining consumed oxygen or liberated hydrogen peroxide. A kit for nucleoside assay is provided, which contains the recited ingredients.
4385112 NUCLEOSIDE PROCESS FOR AND
AND SAME, KIT
Hideo Misaki, Shigeru Ikuta, Kazuo Matsuura, Shizuoka, Japan. assigned to Toyo Jozo Kabushiki Kaisha
Tadashi Kutsusawa, Hideo Shirane, Mikiharu Fujihara, Hideho Hisada. Koganei. Japan, assigned to Fujizoki Pharmaceutical Co. Ltd
An apparatus for automatically performing a sandwich-type enzyme immunoassay. The apparatus includes a rack for holding test tubes. In the test tubes are beads which provide surfaces for the immunochemical reactions. The rack can be moved in lengthwise and transverse directions. Nozzles for supplying and withdrawing liquids from the test tubes are included. The bead in one tube can be transferred to a second tube. The absorbance of a final liquid is measured spectrophotometrically. and the results are recorded by a printer.
ethyl or are combined as -(CH2)4-, -(CH2)5or -CH2CH2-0-CH2CH2-: R9 is hydrogen, methyl. ethyl M is an integer from 0 to 7: P is an integer from I to 3; AAn is an amino acid chain of from one to three amino acids; N is I or I. 2 or I. 2. 3; when P is I. AAn is AA I; when P is 2. AAn is AA I-AA2: when P is 3. AAn is AA I-AA2-AA3; AA I is glytine or alanine; AA2 is glycine or alanine; AA3 is leucine. glutamine or isoleucine. A method of reducing the adverse effects of mammalian collagenase in a mammalian host in need thereof. which comprises administering to the mammal an effective amount of a compound having the above formula is within the scope of the invention. 4381344 PROCESS
FOR PRODUCING DEOXYRIBOSIDES USING BACTERIAL PHOSPHORYLASE Janet L. Rideout. Thomas A. Krenitrky. assigned to Burroughs Wellcom Co
4382081 INHIBITORS OF MAMMALIAN COLLAGENASE
Joseph E. Sundeen. lamara Dejneka. assigned to E.R. Squibb Rr Sons Inc.
R-S-CHI-CH-C-(M,),-NH~~-CHRIRI Mammalian collagenase is inhibited by compounds of the formula or salts thereof. wherein R is hydrogen. alkanoyl of 2 to IO carbon atoms or arylcarbonyl: RI is of 3 to 8 carbon atoms. cycloalkyl of 3 to 7 carbon atoms. aryl or arylalkyl: CN. OCO. R5HCRh. R8NR7. OR9. Cl.. Br or R4 is hydrogen. methyl, ethyl, or R5 and R6 are each independently selected as -0CH3 or 4ICH2CH3orarecombined as-OCH2 CH20or -O-(CH2)-O-; R7 and RX are each independently selected as hydrogen, methyl or
7 he novel compounds 3-deala-2’-deoxyadenosine and certain of its derivative\ and their pharmaceutically acceptable salts have anti-inflammatory activity as well as immune response supression activity. 3-dea7a-2’deoxyadenosine and certain of its intermediates are synthesixd by the emyme catalyfed reaction of the appropriately substituted 3-dearapurine with a 2’-deoxyribose donor.