RADIOISOTOPICASSAY USING ISOTOPE TRANSFER TO CHELATOR-TARGET RECOGNITION MOLECULE CONJUGATE
SYSTEM FOR EFFICIENT ISOLATION OF GENES USING PROBES
Jannis Stavrianpoulos assigned to Enzo Biochem Inc A method of forming a therapeutic or diagnostic agent labeled with a radioactive metal ion, which comprises: contacting an unlabeled therapeutic or diagnostic agent, consisting of a substantially non-metal chelating portion and a chelating portion capable of chelating with the radioactive metal ion, with an ion transfer material having the radioactive metal ion bound thereto and having a binding affinity for the radioactive metal less than the binding affinity of the chelating portion for the radioactive metal ion, wherein prior to contacting the chelating portion is unchelated or is chelated with a second metal having a binding affinity with the chelating portion less than the binding affinity of the radioactive metal ion, whereby a radiolabeled therapeutic or diagnostic agent is formed by the contacting, and separating the radiolabeled therapeutic or diagnostic agent from the ion transfer material, is disclosed along with various components and kits useful in practicing this method and several variations thereof. 4772553 METHOD FOR DETERMINATION OF ENZYME ACTIVITY Setsuro Fujii, Satosh Sugiyama, Syouzou Sawai, Toyonaka, Japan assigned to Torii & Co Inc This invention relates to a method for determining an enzyme-, an enzyme inhibitor-, an enzyme activator-, or zymogen-activity, which comprises allowing the enzyme to react with a substrate of the formula See Patent for Chemical Structure (I) (wherein RI represents an amino acid of an oligopeptide combined in the form of ester through the C-terminal carboxyl group of said amino acid or oligopeptide and R2 represents a hydrogen or bromine atom) to hydrolyze the substrate, then allowing the hydrolysis product of the formula See Patent for Chemical Structure to react with a FR-ITR salt (Fast Red-ITR salt (N, N'-diethyl-4methoxymetanilamide diazonium salt)) to form a pigment, and determining the pigment.
Ronald W Davis, Richard A Young assigned to The Board of Trustees of the Leland Stanford Jr University Systems are provided for the dual purpose of cloning genes using antibodies as probes and isolating unknown proteins encoded by cloned DNA. The method employs a vector derived from phage which is used in combination with a high frequency lysogenic host. The vector is further characterized by having an inducible promoter regulating expression ofa gene into which the foreign DNA may be introduced to produce a fused protein, and controlled induction of the prophage with rapid increase in copy number and high level transcription of foreign DNA. The technique is exemplified with a specific lambda phage construct in conjunction with the beta-galactosidase structural gene lacZ. 4788137 DETECTION OF ACTIVATED T-CELLS Ellis L Reinherz, Andres Alcover, Michael J Weiss assigned to Dana-Farber Cancer Institute Inc A method of detecting activated T-cells in a population of cells, the method involving determining intracellular calcium ion concentration or intracellular pH in the cells, a calcium ion concentration two-fold or greater above that of resting T-cells, or a pH increase of over 0.2 pH units, indicating activation.
4788146 LIQUID LOADED PAD FOR MEDICAL APPLICATIONS David F Ring, Wilson Nashed, Thurman Dow assigned to Johnson & Johnson Patient Care Inc Liquid loaded pads useful as wound and burn dressings are prepared from pellicles of microbially-produced cellulose obtained, for example, by culturing Acetobacter xylinum. A pellicle having a thickness from about 0.1 to 15