AAE Abstracts of Papers
Vol. 19, No. 4, April 1993
(Abstract #58, continued)
(Abstract #61, continued)
Both groups repaired with amalgam alone or plaster of Paris and amalgam showed dye leakage of 100% of the perforation wall. The group repaired with plaster of Paris and glass ionomershowed a mean of 72.97% while the perforations repaired with glass ionomer alone showedameanof 21.92%. UsingplasterofParismatrixdidnotimprove the sealing ability of amalgam whereas its use was found to decrease the sealing ability of glass ionomer. It could controt the overextension of amalgam and glass ionomer cement when used for the repair of furcation perforation.
root-end fills were placed in a blind fashion, consisting of either Diaket or 2 parts Diaket mixed with one part tricalcium phosphate (D/TPO4) and the root-ends were burnished with 10% citric acid for 2 minutes. Followingsacrifice at 30 & 60 days, the animals were perfused with 10% NBF, the jaws were resected and sections were fixed, demineralized and prepared for light microscopy, using serial sections cut at 2p and stained with H&E. Specific evaluation included degreeof inflammation, connective tissue response, periodontal ligamentformation and bone formation/apposition. Sections were graded by two investigators in a blind fashion, with the course of wound healing scored as being < 50%, 50%, or complete. Scores were subjected to the Chi-square test for analysis. In both groups, 30 days specimens showed variable inflammation, encapsulation of the root-end filling material and initiation of functionally oriented PDL fibers. 60 day specimens showed a similar, but advanced healing response. For all factors evaluated, only the degree of cementum deposition at 60 days on the D / T P O 4 specimens showed any significant difference. It is concluded that the healing of the periradicular tissues is comparable in the presence of both root-end filling materials at 60 days. Supported by Baylor College of Dentistry Research Award and Research & Education Foundation of the AAE.
Abstract #59 - Sealing ability of a mineral trioxide aggregate in lateral root perforations. A. Seluti*, S.J. Lee, M. Torabinejad Loma Linda University, Loma Linda, California A number of materials with varying degrees of success have been used to repair tooth perforations. The purpose of this study was to compare the sealing ability of a mineral trioxide (MT) aggregate with those of amalgam and IRM for repair of experimentally induced root perforations. Fifty sound human extracted mandibular and maxillary molars were used in this study. A perforation was made with a#80 file on the mesial root surface at about 45 degree angle to the Iong axis of each tooth. The teeth were then placed into a saline-soaked "Oasis" to cover their perforations and simulate clinical conditions. After placing the repair materials into the perforations (15 teeth~material), the teeth were kept in 100% humidity for 4 weeks. The perforations in five teeth were not repaired and served as positive controls. The perforation sites were then stained with methylene blue for48 hours, sectioned, and examined under adissecting microscope. The amount of linear dye penetration was measured and statistically analyzed using the two-way analysis of variance. TheresultsshowedthattheMTaggregatehadsignificantly less leakage than IRM or amalgam (p < 0.05). In addition, the MT aggregate also showed the least overfilling tendency while l RM showed the least underfilling tendency. Abstract #60 - Furcation perforations repaired with light and chemical cured materials. V.T. Himel*, H.A. Alhadainy University of Tennessee, Memphis, Tennessee A perforation defect into the furcation area usually leads to failure. This studywas an attempt toflnd a material and atechnique which can repair this defect. Two light cured materials, Vitrebond and Prisma VLC Dyca~,were compared to two chemicallycured materials, Ketac Fil and Dycai, for their ability to seal furcation perforations. Access openings and furcation perforations were prepared in 60 teeth and randomly divided into 4 equal groups. Furcation perforations were repaired with each of the tested materials and the access openings were filled with composite resin. After teeth were immersed in 2% erythrocine B dye solution for ten days, they were sectioned longitudinally and dye penetration was measured. The percentage of dye penetration in relationto the perforation walls in each section was calculated for each group. The chemically cured Dycal showed the highest percentage of dye penetration with a mean of 93.01% followed by Ketac Fil with a mean of 67.94%. Light cured Prisma VLC Dycal showed a mean of 33.19% while Vitrebond was found to have the lowest dye penetration with a mean of 26.48%. The light cured materials allowed statistically significant less dye leakage than did the chemically cured materials. Abstract #61 - Healing response to diaket-tricalcium phosphate root-end fillings. S.S. Williams*, J.L. Gutmann Baylor College of Dentistry, Dallas, Texas The short-term healing of the periradicular tissues in response to Diaket and Diaket with tricalcium phosphate as root-end filling materials was determined. Seven mongrel dogs received nonsurgical root canal treatment on their 3rd and 4th mandibular premolars. Subsequently the root-ends were resected, preparations were made in the root-ends,
Abstract #62 - Detection of HIV in human periapical lesions. J.D. Kettering*, M. Torabinejad, J.J. Rossi, D.A. Elkins Loma Linda University, Loma Linda, California and Beckman Research Institute, Duarte, California Dental pulp from a patient with aids (PWA) has revealedthe presence of proviral HIV DNA. The purpose of this study was to determine the feasibility of PCR amplification to detect the H IV proviral DNA in cells from periapical lesions from a PWA, when compared to similar cells from an HIV-negative patient who required endodontic surgery. Approximately 50 ng of extracted DNA solution were used for each PCR. Two primer pairs were used:one within the env coding region and the other within the gag coding region. Amplification proceeded through 30 cycles. Samples of each reaction were separated by non-denaturing PAGE and stained with ethidium bromide (EtBr) for visualization. Gels were electroblotted to nylon membranes, which were then fixed, denaturedand dried. Membraneswere hybridizedto specific radioactive oligonucleotide probes and placed next to Kodak XAR film for visualization of the HIV-specific bands. No evidence of HIV-specific reaction was observed in L929 cells (negative control) and a periapical lesion from an HIV-negative patient. The EtBr strains revealed that DNA extracts from two lesions from the PWA yielded PCR bands (with both primer pairs) which corresponded to HIV-specific bands of the expected size. The presence of back-ground bands prevented these results from being conclusive, although hybridization with probes internal to the primer sequences allowed us to discriminate between background and target bands. This represents the first report of HIVproviral DNA found in periapical lesions material. Abstract #63 - Effectiveness of various medications on pain following instrumentation. M. Frankson*, J. Cymerman, R. Lemon, H. Schilder, M. Torabinejad Loma Linda University, Loma Linda, California Several medications have been used to reduce pain following instrumentation. The purpose ofthis prospective study was to compare the effectiveness of nine medications in controlling pain following complete cleaning and shaping of root canals. Five hundred eighty eight patients who required endodontic therapy were included in this study. After complete cleaning and shaping of root canals, each patient took one of the nine medications (analgesic, antibiotic, analgesic and antibiotic) or a placebo encapsulated in identical capsules every six hours for 72 hours. The patients registered their degree of discomfort on a visual analogue scale of 0-9 for 72 hours. Statistical analysis (ANOVA and McNemar) showed that Ibuprofen (400 rag), Ketoprofen (50 mg), Eryc (500 mg), penicillin (500 mg) and penicillin (500 mg) plus methylprednisolone were statistically more effective than placebo in