24, 145-146 (1982)
Manual of Macrophage Methodology: Collection, Characterization and Function. Edited by H. B. HERSCOWITZ, H. T. HOLDEN. J. A. BELLANTI. AND A. GHEFFAR. Immunology Series, Vol. 13. Marcel Dekker, New YorWBasel, 1981. 530 pp. Investigation of Phagocytes in Disease. edited by S. D. DOUGLAS AND P. G. QUE. Practical Methods in Clinical Immunology Series, Vol. 3. Churchill-Livingstone, Edinburgh/Melbourne/New York, 1981. 79 pp.
These are two very well written, comprehensive, and useful volumes of current methodologies on how to handle phagocytes, what tests to perform with them, and how to interpret their results. Each method has been written by experts in the field who obviously have firsthand knowledge of the subject matter and can advise the novice. The methods are easy to follow and are as foolproof as laboratory methodologies can be expected to be. Many have been tried successfully in this reviewer’s laboratory. The Manual of Macrophage Methodology is the larger of the two volumes (530 pages) and deals with (monocytes and) macrophages only. The volume is divided into four parts and comprises 58 methods: the four parts are (1) collection and separation, which is subdivided into source, enrichment, depletion by physical methods and by toxic agents, and culture techniques: (2) characterization; (3) in Ivitro functional activity; and (4) in viva functional activity. Investigation oj Phagocytes in Disease is a smaller volume (79 pages) of 11 selected chapters dealing with leukocytes, granulocytes, polymorphs, and monocytes. Both volumes are excellently presented, well illustrated, and referenced. Both books are well indexed. These two volumes can be enthusiatically recommended for any laboratory dealing primarily with in vitro methodologies for the study of phagocytes and their functions, Ezro
and Clinical Immur~olo,~~ and Microbioloy~ Medical fJni\wsiry of South Carolintr Charleston. South Carolina 29425
Apheresis: Development, Applications, and Collection Procedures. Progress in Clinical and Biological Research, Volume 65. Edited by C. HAROLD MIELKE. JR. Alan R. Liss, Inc., New York, 1981. 168 pp., $18.00.
Plasmapheresis and leukapheresis as therapeutic modalities have attracted considerable attention from clinicians in recent years. Manual plasmapheresis has 145 0090-1229182/070145-02$01.00/O Copyright @ 1982 by Academic Press. Inc. All rights of reproduction in any form reserved.
been used for the treatment of hyperviscosity syndromes for more than 50 years, but it was not until the development of mechanical cell separators-continuous flow and intermittent flow centrifuges-that the possibility of other clinical applications began to be recognized. This volume, although it does not give a really comprehensive review of newer therapeutic uses of apheresis techniques, provides a springboard from which the interested reader can launch his exploration of this fast-growing area of clinical investigation. The book focuses on the collection of granulocytes and cell platelets, as well as therapeutic applications of plasma and cell pheresis techniques. The opening and closing chapters by Dr. Emil Freireich, a pioneer in the technology of mechanical cell separation, are particularly interesting, and discussions of the potential complications that donors may experience and the difficulties actually encountered during apheresis will be of practical value for many readers. H. HUGH
Depurtment oj Basic und Cliniccd Immurzology and Microbiology Medical UnivcJrsity of South Carolina Churleston. South Carolina 29425