Auramine-Rhodamine Stain—Detection of Mycobacteria in Ocular Fluid

Auramine-Rhodamine Stain—Detection of Mycobacteria in Ocular Fluid

Spiteri Cornish et al  Stargardt Disease in the United Kingdom Footnotes and Financial Disclosures Originally received: February 7, 2017. Final re...

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Spiteri Cornish et al



Stargardt Disease in the United Kingdom

Footnotes and Financial Disclosures Originally received: February 7, 2017. Final revision: February 27, 2017. Accepted: March 3, 2017. Available online: April 21, 2017.

Author Contributions: Research design: Spiteri Cornish, Lois Manuscript no. ORET_2017_36.

1 Sheffield Teaching Hospitals NHS Trust, Sheffield, England, United Kingdom. 2

Moorfields Eye Hospital, London, England, United Kingdom.

3

University College London UCL Institute of Ophthalmology, London, England, United Kingdom.

4

Oxford University Hospitals, Oxford, England, United Kingdom.

5

Medical Statistics Team, University of Aberdeen, Aberdeen, Scotland, United Kingdom.

6

Queens University, Belfast, Northern Ireland, United Kingdom.

Financial Disclosure(s): Supported by the Fighting for Sight Aberdeen (Registered Charity SC033004). The funding organization had no role in the design or conduct of this research. Conflict of interest: No conflicting relationship exists for any author.

Data analysis and/or interpretation: Spiteri Cornish, Ho, Downes, Scott, Lois Data acquisition and/or research execution: Spiteri Cornish, Ho, Downes, Scott, Bainbridge, Lois Manuscript preparation: Spiteri Cornish, Lois Abbreviations and Acronyms: AF ¼ autofluorescence; BOSU ¼ British Ophthalmological Surveillance Unit; FFA ¼ fundus fluorescein angiography; FFERG ¼ full-field electroretinography; FFM ¼ fundus flavimaculatus; logMAR ¼ logarithm of the minimum angle of resolution; PERG ¼ pattern electroretinography; PR ¼ photoreceptor; RPE ¼ retinal pigment epithelium; STGD ¼ Stargardt disease; UK ¼ United Kingdom. Correspondence: Noemi Lois, MD, PhD, Wellcome-Wolfson institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queens University Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK. E-mail: [email protected]

Pictures & Perspectives

Auramine-Rhodamine StaindDetection of Mycobacteria in Ocular Fluid A 38-year-old male patient presented with a large subretinal mass in the left eye. There was massive exudation around the lesion and at the periphery of the fundus (Fig 1A). The right eye was normal. A provisional diagnosis of choroidal granuloma due to tubercular etiology was made. A biopsy sample of the vitreous humor was subjected to various investigations. An auramine-rhodamine stain of vitreous fluid showed acid-fast organisms exhibiting bright orange fluorescence against a dark background, confirming our diagnosis (Fig 1B). (Magnified version available at www.ophthalmologyretina.org).

PRIYA BAJGAI, MS SURYA PRAKASH SHARMA, MSC RAMANDEEP SINGH, MS Advanced Eye Centre, Postgraduate Institute of Medical Education and Research, Chandigarh, India

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