Flow Cytometry — Clinical Applications

Flow Cytometry — Clinical Applications

Pathology (1996),28, pp. 110-111 BOOK REVIEWS Common Problems in Pediatric Pathology. By VUAY V. Butterworth-Heineman, Sydney, 1993. ISBN 0896402428...

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Pathology (1996),28, pp. 110-111


Common Problems in Pediatric Pathology. By VUAY V. Butterworth-Heineman, Sydney, 1993. ISBN 0896402428, pp. xi 524. A$315.00.


As its title indicates this is not intended to be a comprehensive textbook of pediatric pathology. Yet in a practical way this book surpasses some pediatric pathology textbooks in usefulness because of the thoroughness with which Dr loshi has addressed the problem areas in fetal and neonatal pathology, developmental anomalies, metabolic defects, infections, neoplasia, iatrogenic and other acquired disorders in infancy and childhood. Without purporting to have the answer to every problem, the 13 well set out chapters are full of up-to-date information on the subject matters and helpful practical guidelines on the handling of actual specimens to facilitate problem solving, with excellent bibliographies. The style is highly readable. I found this book totally absorbing and the few slightly suboptimally reproduced photomicrographs did little to diminish my pleasure in reviewing it. Now I cannot imagine being without it. Alex E. Kan Department of Histopathology Royal Alexandra Hospital for Children, NSW Fetal Pathology. By JEAN W. KEELING. Churchill Livingstone, New York, 1994. ISBN 0-443-04426-0, pp. viii + 164. A$257.00. Over the past 5 to 10 years there has been a rapid growth of knowledge and interest in the field of perinatal pathology consequent on the increased use of high resolution ultrasound screening and the availability of other forms of prenatal diagnosis. The increasing ability of our ultrasound colleagues to identify subtle fetal abnormalities has led to a change in the population of fetuses and infants that come to post mortem. Fetuses under the age of 20 weeks of gestation now make up a large proportion of autopsy cases that are submitted for confirmation of prenatally diagnosed anomalies. Early stage fetuses frequently do not display the classic fully developed features of the many syndromes and disorders that are required to be evaluated. A further problem is that people who are unfamiliar with the normal appearances ofearly gestation fetuses, and the changes that normally follow fetal death in utero, may attribute some of these appearances to pathological abnormalities. The standard texts do not in general emphasize the specific and often subtle diagnostic features related to this group of infants and there has been a need for a detailed atlas or bench side text to fill this gap. In response to these needs, Dr Jean Keeling, doyenne of perinatal pathology, has published this text containing numerous colour macrophotographs of a great variety of fetal abnormalities. The book is prefaced by helpful notes on the conduct of a perinatal autopsy and illustrates some iatrogenic and degenerative features that may confuse the newcomer to perinatal pathology. The book is divided into chapters dealing firstly with the common group of chromosomal anomalies and then into abnormalities affecting regional anatomical sites. Each section is accompanied by brief explanatory notes and tables. The appendices which include detailed anthropomorphic tables and placental and umbilical cord measurements, are particularly useful as they concentrate on the first and mid-trimester fetus. These data have only recently been well documented and available. The colour photographs are generally of high quality and clearly demonstrate the diagnostic features. There are however up to 15 plates that are poorly focused and show excessive highlights and reflections that mar the overall presentation of the book. This book is a useful addition to the classic texts on infant and perinatal pathology. It is recommended for those practices where trainees are taught or where perinatal autopsies are infrequently performed. Virginia Billson Department of Anatomical Pathology The Royal Women's Hospital, Vic

Flow Cytometry - Clinical Applications. Edited by MARION G. Blackwell Science, Oxford, 1994. ISBN 0-632-03673-7, pp. xii + 308. Price NI A.


While still a technique with considerable relevance to research, flow cytometry is increasingly becoming a routine laboratory tool. This volume is an excellent introduction to the subject as well as serving as an informative reference source. The book begins with very useful chapters on introduction and quality control, and then goes on to describe the use of flow cytometry in analysing leucocytes, platelets, and red cells, as well as DNA and chromosome analysis. Dr Macey, as editor, concludes the book with a valuable review of developing techniques. There are many useful aspects to this book. The methods are concisely described and presented, with highlighting in a way that makes them very easy to follow. The CD classification - the arcane language of those familiar with flow cytometry - is well summarized in tables and updated in an appendix reporting the deliberations of the Vth International Workshop (held in November 1993). The various dyes are well described in detail, and the different analysers and sorters are compared in an excellent table in the Introduction. This book is a useful introduction for the novice and an invaluable reference for the expert in flow cytometry. It is a must for anyone in the field. Paul C. Vincent The Kanematsu Laboratories Royal Prince Alfred Hospital, NSW Paediatric Surgical Pathology. By E. S. GRAY AND N. SMITH. Churchill Livingstone, Edinburgh, 1995. ISBN 0-443-04122-9, pp. viii + 204. A$195.00. This illustrated hardback on pediatric surgical biopsy pathology has been written by Drs Elizabeth Gray and Nicholas Smith of Aberdeen and Edinburgh respectively, although the authors acknowledge the contribution of material from many of their colleagues. As indicated in the preface, the book is by no means comprehensive. It aims only to provide a practical illustrated first-line guide to the diagnosis of surgical biopsy material. It does not attempt to cover the highly specialized areas of neuropathology and bone pathology and, while congenital conditions are emphasized, metabolic and storage diseases are only mentioned where biopsy may be performed such as in the liver. In other words, the book illustrates the surgical biopsy pathology that may cross the desk of the pediatric and adult pathologist in a routine reporting day. The 12 chapters discuss the pathology in a systematic way as well as covering specific topics such as muscle, soft tissue lesions and small cell tumors of childhood. The full colour photographs are of good technical quality and illustrate well the macroscopic and microscopic pathology accompanying the concise text. Where appropriate, molecular biology and immunohistochemistry are included, in particular in relation to tumors. The recommended further reading at the end of each chapter includes not only traditional main pediatric pathology texts but also helpful more recent literature references. There is also some clarification of difficult areas such as the current classification of histiocytoses syndromes and congenital anomalies of the testis. Appendices illustrate protocols for handling fresh biopsy tissue. Within the restricted framework set by the authors, this book is concise yet informative, well written and illustrated and it bridges the gap between standard histopathology texts and pediatric pathology texts. I recommend it as a basic diagnostic and reference guide to registrars and to training and anatomical pathology departments who deal with any pediatric surgical biopsies. Claire Cooke-Yarborough Department of Histopathology The Royal Children's Hospital, Vic