Fluorescent and MRI bimodal imaging agent for tumor hypoxia

Fluorescent and MRI bimodal imaging agent for tumor hypoxia

506 ChinaNanomedicine Abstracts / Nanomedicine: Nanotechnology, Biology, and Medicine 12 (2016) 449–575 (TEM) and low-temperature magnetic measureme...

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ChinaNanomedicine Abstracts / Nanomedicine: Nanotechnology, Biology, and Medicine 12 (2016) 449–575

(TEM) and low-temperature magnetic measurements. Results demonstrated well crystalline magnetite cores within intact ferritin shells. The saturation magnetization (Ms), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores. The M-HFn nanoparticles with largest core size of 5.3 nm exhibit the strongest saturation magnetization, the highest peroxidase activity in immunohistochemical staining, and the highest r2 of 321 mM− 1 s− 1, allowing to detect MDA-MB-231 breast cancer cells as low as 104 cells ml− 1. This study indicates that M-HFn nanoparticles with larger ferrimagnetic core can significantly enhance the performance in MRI and staining of cancer cells.


Fluorescent and MRI bimodal imaging agent for tumor hypoxia Qi Cai, Juan Zhou, Weiping Zhu⁎, Yufang Xu, Xuhong Qian, Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, China ⁎Corresponding author. E-mail address: [email protected] (W. Zhu) Hypoxia is one of the essential features in human solid tumors, which causes resistance to traditional radiotherapy, phototherapy, chemotherapy and conventional cancer immunotherapy. Recently, magnetic nanoparticles play an emerging role in magnetic resonance imaging accompanied by controlled release of drug molecules. In addition, fluorescent probe has gained popularity on the detection and imaging of tumor hypoxia with high sensitivity, good spatial and temporal resolution. Herein, we combined magnetic mesoporous silica nanoparticle with “off–on” hypoxic probe for dual-mode imaging of tumor. We selected 9-nitro-acenaphtho[1,2-b]quinoxaline as the hypoxia responsive probe, and then connected the functionalized quinoxaline with magnetic nanoparticle to build the magnetic mesoporous silica nanoparticle F8, which was then modified by folic acid and polyethylene glycol (PEG) on the surface. The results show that the mesoporous silica distributes around 100 nm size with high magnetic performance. The fluorescence differences are significant between SiHa cells incubated under aerobic and hypoxic with these nanoparticles separately. The results demonstrated an innovative approach to the development of a novel dual-mode imaging agent for solid tumors.

Figure 1. (a) The model of target magnetic mesoporous silica nanoparticle F8. (b) The image of magnetic nanoparticle F8 traveling across the cuvette, propeled by magnetic force. (c) T2weighted MR images of three concentrations of magnetic nanoparticles. (d and e) Fluorescence microphotographs of SiHa cells incubated with 0.05 mg/mL of F8 at 37 °C for 5 h. (d) SiHa cells on aeroxic condition (incubated in air and 5% CO2). (e) SiHa cells on hypoxic condition (incubated in nitrogen and 5% CO2).

We acknowledge the financial support from the National Natural Science Foundation of China (Grants 21476077) and Shanghai Pujiang Program.


Fluorescent nanoparticles for drug delivery and theranostic Xingang Guan, Zhigang Xie⁎, Xiabin Jing, Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, China ⁎Corresponding author. E-mail address: [email protected] (Z. Xie) Polymeric nanoparticles have been of significant interest for application in medical diagnosis and therapeutics. Recent clinical studies have proved that polymeric NPs incorporating anticancer drugs will lead to better tumor regression compared to free drugs. Fluorescent molecules are suitable models to prove the successful encapsulation and to allow for tracking the fate of nanoparticles in vivo. Recently, we have developed several fluorescent nanoparticles and studied their application in bioimaging and theranostic agent for personalized medicine. For example, near-infrared fluorescent nanovesicles were prepared by self-assembly of block copolymer. As shown in the figure below, the fluorescence enhancement induced by dissociation of nanovesicles could be used as a smart imaging and diagnostic tool. These nanovesicles could encapsulate the antitumor drug, and provide a powerful platform for imaging-guided tumor-specific drug delivery and therapy.


Fluorescent zinc doped carbon nanodots derived from chitosan/metal ions complex for cell imaging Yinfeng Cheng, Baoqiang Li⁎, Lei Wang, Daqing Wei, Yujie Feng, Dechang Jia, Institute for Advanced Ceramics and State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China ⁎Corresponding author. E-mail address: [email protected] (B. Li) Fluorescent carbon nanodots (CNDs), with excellent luminescent properties and low toxicity, are attractive for cell imaging. The emission spectrum of CNDs could be modulated by control of sizes and doping nitrogen elements. Herein, we firstly reported the synthesis of zinc doped CNDs (Zn-CND) with tunable emission spectrum derived from chitosan/zinc ions complex via hydrothermal treatment. The photoluminescence (PL) spectrum (380-500 nm) of Zn-CNDs was shown in Figure 1, A; the λexdependent emission confirmed the presence of Zn-CNDs. Compared with CNDs directly derived from chitosan, the maximum excitation wavelength of Zn-CNDs shifted from 380 nm to 420 nm and the maximum emission wavelength shifted from 469 nm to 509 nm (Figure 1, B) as well as accompanying with enhanced PL intensity (29.3%). Under UV light, slight yellow Zn-CNDs solution emitted bright blue color (Figure 1, C). The green fluorescence could be observed in HCT116 colorectal cancer cells (Figure 1, D). Zn-CNDs with tunable fluorescent properties derived from chitosan/zinc ions complex exhibited potential application in cellular imaging and labeling.

The project was sponsored by NSFC: 51372051, 51321061, 2012CB3393, 2013TS09, 2013RFLXJ023 and HIT.IBRSEM.201302.