associated with higher abundance of Pseudoflavonifractor capillosus (FDR-adjusted P=0.15) and Lachnospiraceae bacterium 1 4 56FAA (FDR-adjusted P=0.16) and lower levels of Coprococcus eutactus (FDR-adjusted P=0.15). Conclusions: Short-term alcohol intake as measured prior to stool sampling and long-term intake over 26 years demonstrated consistent and distinct effects on gut microbial communities, which opens the possibility that these bacteria may mediate, at least in part, the pathogenesis of alcohol associated chronic diseases.
samples were collected and analyzed for metabolomics profiling. Results: Overall 28 patients (male gender: 50%, mean age: 49±13 years, mean pouch age: 12±9 years) were recruited. Of those, 15 had a normal pouch, 12 had pouchitis and 1 - familial adenomatous polyposis. All patients completed both investigated dietary regimens. MED and SCD were isocaloric (Kcal: 1910±575 vs. 1857±614, p=0.4), however diets differed in their macronutrients composition: MED had higher carbohydrate content and lower protein, fat and saturated fat content compared with SCD (carbohydrate, gr/day: 204±66 vs. 96±48; protein, gr/day: 80±26 vs. 119±45; fat, gr/day: 78±29 vs. 105±40; saturated fat, gr/day: 16±10 vs. 34±14; all p<0.001). Fecal metabolomics analysis detected a total of 1051 named biochemicals. Both MED and SCD induced significant changes in 182 and 313 metabolites respectively, including changes in super-pathways associated with carbohydrate, lipid, amino acid, peptide, nucleotide, vitamin and xenobiotics metabolism. MED and SCD had a discordant effect on branched-chain amino acid metabolism. Both diets induced an upregulation of vitamin A metabolism. Interestingly, both diets upregulated mono and diacylglycerol and downregulated endocannabinoids metabolism, however ceramides metabolism was downregulated in MED and upregulated in SCD. Hierarchical clustering analysis showed that samples from each study participant tended to be more similar to each other than to other participants. Of note, Post-diet changes in C reactive protein levels were associated with pre-diet metabolites levels. Conclusions: In this cross-over trial, two unindustrialized dietary interventions (MED and SCD) rapidly altered the metabolomic profile of patients with IBD after pouch surgery, despite individual metabolomic signatures. Changes in these metabolites might alter signaling pathways associated with inflammatory responses such as autophagy and activation of NLRP3 inflammasome. Thus, personalized dietary intervention may be used to modify inflammation in IBD via altering metabolomics profile.
Sa2034 INTRAPERITONEAL TREATMENT WITH KISSPEPTIN MODIFIES FEEDING BEHAVIOR AND CHANGES GASTROINTESTINAL HORMONES IN MICE Tien S. Dong, John P. Vu, Suwan Oh, Daniel Sanford, Joseph R. Pisegna, Patrizia M. Germano Background: Kisspeptin is a neuropeptide integral in the regulation of reproduction and metabolism. But much of the research published has been focused on its central effects on the hypothalamus-pituitary-gonadal axis. We have explored for the first time the effects of a treatment with a single intraperitoneal dose of kisspeptin-10 on the regulation of food and water intake, energy balance, metabolism, and metabolic hormones. Methods: Two groups (n=16) of C57BL/6 wild-type adult mice were individually housed in metabolic cages and intraperitoneally injected with either kisspeptin-10 (2 nmol/200 μl of saline) or control before the beginning of a dark-phase feeding period. Microstructure food and water intake, calorimetric parameters, and plasma metabolic hormones were measured. Results: Intraperitoneal injected kisspeptin-10 induced a significant reduction of food intake (33%; p<0.05), meal frequency (33%; p<0.05), meal size (30%; p<0.05) and eating rate (59%; p<0.05). Kisspeptin-10 significantly decreased total energy expenditure during both dark (0.42 kcal/ hr vs. 0.47 kcal/hr; p=0.02) and light phase cycle (0.31 kcal/hr vs. 0.39 kcal/hr; p<0.001). The respiratory quotient was significantly higher in kisspeptin-10 treated mice during the dark phase (0.81 vs. 0.77 p<0.001). In addition, kisspetin-10 increased plasma levels of insulin (343.8 pg/ml vs. 106.4 pg/ml; p=0.005), leptin (855.5 pg/ml vs. 173.1 pg/ml; p= 0.02) and resistin (9411.1 pg/ml vs. 4116.5 pg/ml; p=0.001). Conclusion: Intraperitoneal kisspeptin-10 decreased food intake, meal size, eating rate, and energy expenditure, without affecting activity levels. These changes were linked to increased plasma levels of insulin, leptin, and resistin. This suggests that kisspeptin regulates appetite/satiety, metabolism, and energy homeostasis.
Sa2036 AN EVALUATION OF DIETARY INTAKE, MACRO AND MICRONUTRIENTS ADEQUACY AMONG PATIENTS WITH CONSTIPATION-PREDOMINANT IRRITABLE BOWEL SYNDROME AND HEALTHY SUBJECTS: REAL WORLD DATA IN MALAYSIA Raja Affendi Raja Ali, Nor Hamizah Shafiee, Norfilza Mood Mokhtar Background/Aim: Substantial proportion of patients with constipation-predominant irritable bowel syndrome (IBS-C) associate their symptoms with the consumption of specific dietary foods which contain different types of macro and micronutrients. Therefore, dietary manipulation is now considered an important aspect in the management of IBS-C. However, little is known about the dietary intake, macronutrients and micronutrients adequacy among IBSC patients prior to dietary intervention.Thus, the aim of this study was to evaluate the dietary intake, macro and micronutrients adequacy among IBS-C patients and to compare to healthy subjects and standard recommended nutrient intake (RNI) for Malaysians. Methods: Patients diagnosed with IBS-C based on Rome III criteria and healthy subjects were invited to participate in the study. All subjects were asked to complete a validated food frequency questionnaire which consists of 241 food types that are based on Malaysian population and guided by dietitian. Data were analysed against Dietary Reference Values for food energy and nutrients for the Malaysians. The observed nutrients intake for IBS-C patients were then compared with the healthy subjects and the standard RNI for Malaysians.The detailed analysis intake of nutrients was calculated using Nutritionist ProTM Diet Analysis 4.0 (Axxya Systems, Woodinville, WA USA) software. Results: A total of 218 IBS-C patients and 88 healthy subjects were evaluated. Details of the baseline characteristic are shown in Table 1. The dietary intakes among IBS-C patients did not meet the standard RNI for Malaysians. The total energy, macronutrients (protein, fat and fibre) and micronutrients (folate, potassium, vitamins B6, D, E and K) intakes among IBS-C patients were significantly lower than the healthy subjects. Details of the nutrients intake are shown in Table 2. Conclusion: In Malaysia, the habitual dietary intakes among IBS-C patients did not meet the standard recommended nutrient intake. There are significant macronutrients (protein, fat and fibre) and micronutrients (folate, potassium, vitamins B6, D, E and K) inadequacies among patients with IBS-C as compared to healthy subjects. Our study emphasized the importance of comprehensive nutrional assessment in patients with IBS-C patients prior to any dietary manipulations. These findings can potentially guide both clinicians and dietitians for the optimal dietary management in IBS-C patients in order to maintain the nutritional adequacy. Table 1. Baseline characteristics of IBS-C patients vs healthy subjects Baseline characteris- IBS-C patients (n= tics 218) Mean Age (years) 31.3 Male 56 Female 162 Malay 150 Chinese 64 Indian 2 Others 2 Single 111 Married 102 Divorced 5 Primary education 7 Secondary educa42 tion Tertiary education 169 Monthly income < 100 RM1500 Monthly income > 118 RM1500 Employed 133 Unemployed 85
Sa2035 DIETARY INTERVENTIONS RAPIDLY ALTER METABOLOMICS PROFILE OF PATIENTS WITH INFLAMMATORY BOWEL DISEASE AFTER POUCH SURGERY Lihi Godny, Leah Reshef, Tamar Pfeffer-Gik, Keren M. Rabinowitz, Idan Goren, Karin Yadgar, Keren Zonensain, Revital Barkan, Henit A. Yanai, Uri Gophna, Hagit Tulchinsky, Iris Dotan Background: Diet may play a role in the pathophysiology of inflammatory bowel diseases (IBD) via several mechanisms including metabolome alteration. We conducted an interventional cross-over study aiming to evaluate the short-term effect of two dietary regimens, the Mediterranean diet (MED) and the specific carbohydrate diet (SCD) on multiple biomarkers of patients with IBD after pouch surgery. Methods: Two short (one-week) dietary interventions (MED and SCD) were provided to patients. After one washout week, patients were crossed over between diets. Both diets excluded industrialized and processed food. Fecal
Healthy subjects (n= 88) 29.3 38 50 72 16 0 0 51 37 0 0 28
p-Value 0.127 0.004 0.108 0.233 0.02 -