Method for construction of normalized cDNA libraries

Method for construction of normalized cDNA libraries

Patent reports ide, and having a pH of at least 8.5 such that the aqueous solution is completely absorbed by the plant material without establishing a...

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Patent reports ide, and having a pH of at least 8.5 such that the aqueous solution is completely absorbed by the plant material without establishing any liquid phase; (2) heating the soaked plant material at a temperature ranging from 4&lOO”C for a period of time sufficient to effect the bleaching, washing the bleached plant material with water, and then drying it.

Immunology Antibodies to Complexes of Ligand Receptors and Ligands and Their Utility in Ligand-receptor Assays Buechler et al. Biosite Diagnostics, Inc. US 5480792; January 2, 1996 Methods and test devices for detecting the presence or amount of target ligand in noncompetitive sandwich ligand-receptor assay processes. Antibodies which bind to the complex of ligand receptor and target ligand but do not bind significantly to the ligand receptor and which bind the target ligand with substantially less affinity than the complex are described. Hybridomas and Monoclonal Antibodies which Specifically Bind Human Basic Fibroblast Growth Factor Igarashi et al. Takeda Chemical Industries Ltd. US 5478740; December 26, 1995 A monoclonal antibody produced from a cloned hybridoma which combines specifically with basic fibroblast growth factor (bFGF). The monoclonal antibody can be advantageously used for assay reagents on bFGF or for purification of bFGF.

Preserved Cell Preparations for Flow Cytometry and Immunology Caldwell University of Missouri US 5478722; December 26, 1995 Cells treated for preservation in a nonfrozen hydrated form with minimal alterations for their antigens or nucleic acids. This makes the cells useful as quality control reagents for processes such as calibrating and standardizing flow cytometry equipment, for use as “unknown” test samples for quality control testing programs, and to prepare patient specimens for archival storage.

This invention takes advantage of the discovery of a positionspecific endonuclease and position-specific insertion markers for the design of the vectors.

Nucleic Acid Encoding TGF-Beta and Its Uses Derynck et al. Genentech. Inc. US 5482851; January 9, 1996 Nucleic acid encoding TGF-beta isolated and cloned into vectors which are replicated in bacteria and expressed in eukaryotic cells. TGF-beta is recovered from transformed cultures for use in known therapeutic modalities.

Method for Construction of Normalized cDNA Libraries Efstratiadis et al. Columbia University US 5482845; January 9, 1996 A method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form. Methods of Identifying Lymphocytes with Mutator Phenotypes Albertini University of Vermont US 5482837; January 9, 1996 Methods for identifying lymphocytes with a mutator phenotype by isolating lymphocytes bearing a selectable marker; identifying among the lymphocytes a population belonging to the same clonally amplified set; and determining whether the population has at least two lymphocytes having between them two independent mutations among different alleles at the gene locus of the selectable marker, the presence of which indicates a mutator phenotype.

Augmenter of Liver Regeneration (ALR) Francavilla et al. University of Pittsburgh and Toyo Boseki KK US 5480797; January 2, 1996 A full-length cDNA clone encoding a purified augmenter of liver regeneration (ALR) polypeptide prepared from the cytosol of livers from weanling rats. The 1.2 kb cDNA includes a 299 bp 5’untranslated region, a 375 bp coding region, and a 551 bp 3’untranslated region. The cDNA encodes a protein consisting of 125 amino acids.

Genetics Pharmaceuticals

Production of Chimeric Antibodies by Homologous Recombination

Trypsin-Sensitive Agent Able to Reduce PAI- and Beta APP Expression in Senescent Cells and Increase the Ability of Fibroblasts to Divide in Culture West

Fell et al. Oncogen, Inc. US 5482856; January 9, 1996 A process for producing chimeric antibodies using novel recombinant DNA vectors and homologous recombination in vivo. The recombinant DNA constructs of the invention can be used to transfeet antibody-producing cells so that targeted homologous recombination occurs in the transfected cells leading to gene modification and the production of chimeric antibody molecules by the transfected cells.

Geron Corporation US 5482838; January 9, 1996 Purified trypsin-sensitive agent or analog thereof, able to downregulate collagenase, PAI- and beta APP expression in senescent cells isolated from culture medium having growing fibroblasts or U937 promyelocytic cells.

Position-specific Insertion Vectors and Method of Using Same

Transmucosal Therapeutic Composition Igari et al.

Sandmeyer University of California US 5482853; January 9, 1996 Vectors for the efficient and position-specific integration of expressible, exogenous nucleotide sequences into cellular genomes.

Takeda Chemical Industries Ltd. US 5482706; January 9, 1996 A transmucosal therapeutic composition comprising a physiologically active peptide or protein and a cytidine nucleotide derivative. The above composition results in a satisfactory transmucosal ab-


Enzyme Microb. Technol.,

1997, vol. 20, February