Friday, 28 May 1999 Poster presentation: Diet and cardiovascular disease Group 1). BMI had tendency to be higher in the Group 3 when compared to other Groups. W/H ratio was the highest in the Group 3. Conclusion: Atherogenic lipid profile of patients is associated with high animal fat frequency intake, high values of BMI and W/H ratio reflecting abdominal type of fat distribution. Ch
BMI, kg/m 2
p < 0.05 compared with Group 1
MODERATE RED WINE CONSUMPTION IMPROVE ENDOTHELIAL FUNCTION IN HEALTHY MEN A. Cuevas, A. Germain, V. Iribarra, C. Mizon, O. Castillo, A. Maiz, F. Leighton. School of Medicine and Biological Sciences, Pontificia
Uniuersidad Catlica de Chile, Santiago, Chile Endothelial dysfunction is an early event in atherosclerosis. In vitro, red wine induces an endothelial dependent relaxation (EDR). To our knowledgement there are no reports evaluating this effect in humans. We evaluate the effects of one month red wine consumption on EDR in men that consume two differents diets. Twelve healthy men received Mediterranean or high fat diets during two months. At the first month they drink 200 ml. of red wine daily. At the second month they did not drink alcohol. Endothelial function was assessed by flow mediated dilation (FMD) in brachial artery after the first and second month of the protocol. After wine consumption FMD was normal in all the subjects, independent of their diet (6.2 + 1.0%). After wine suspension, subjects that received the high fat diet had an abnormal dilatory response to ischemia (-1.0+20%, compared to 6.6+0.8% in the period plus wine; p < 0.05). Individuals receiving Mediterranean diet had a normal endothelial function, independent of the wine status. No significant changes were detected in plasma glucose and lipid levels, blood pressure and body weight. We conclude that moderate red wine consumption improves endothelial function mainly in individuals that consume a high fat diet. Antioxidants o f the red wine may mediate this effect. SOY PROTEIN IMPROVES ENDOTHELIAL DYSFUNCTION IN POSTMENOPAUSAL WOMEN R.J. DuBroff, PJ. Decker, W.A. Gray. Southwest Cardiology Associates,
Albuquerque. NM. USA The purpose of this study was to determine if isoflavone containing soy protein can improve endothelial dysfunction in postmenopausal women. Endothelial dysfunction occurs commonly in postmenopausal women and is reversible with exogenous estrogen. Nineteen of thirty one postmenopausal women not taking estrogens, anti-estrogens, cholesterol lowering medications, or vitamins were found to have abnormal (<6%) endothelial dependent dilation o f the brachial artery (mean 2.08% +/- 1.79). Repeat brachial artery testing one month after daily consumption of a 40 gram soy protein beverage demonstrated a significant improvement (mean 5.82% +/- 4.06, p = 0.004). Five subjects dropped out o f the study, three for soy intolerance. This study demonstrates a significant normalizing effect of soy protein upon endothelial dysfunction in postmenopausal women. Soy product and financial support was provided by Protein Technologies International.
m.~12 ~ - z 10
TREATMENT DURATION (MONTHS)
THE IMPACT OF DIETARY FATTY ACIDS ON SUSCEPTIBILITY OF LOW-DENSITY LIPOPROTEIN TO OXIDATION M. Kratz I , P. Cullen t.2, A. KassnerI , M. Fobker2, P.M. Abuja 3, H. Schulte t , G. Assmann 1'2, U. Wahrburg t . /Institute of Arteriosclerosis
Research at the University of Miinster; 2Institute of Clinical Chemistry and Laboratory Medicine, Uniuersity of Miinster, Germany; 3institute of Biochemistry, Uniuersity of Graz, Austria Objective: The oxidation of low-density lipoprotein (LDL) is thought to play a crucial and causative role in atherogenesis. This study aimed to investigate the effects o f diets rich in monounsaturated fatty acids (MUFA), to-6 and (0-3 polyunsaturated fatty acids (PUFA) on the in vitro oxidizability of LDL. Methods: We conducted a strictly controlled dietary study with 56 healthy young volunteers. In the wash-in phase the participants received a saturated fatty acid (SAFA)-rich diet for 2 weeks. Then we administered in a parallel design three diets containing either refined olive oil, sunflower oil, or rapeseed oil as the principal source of fat for 4 weeks. Intake of all other nutrients, especially antioxidants, was similar in all three groups. Susceptibility of LDL to oxidation was assessed by measuring the formation of conjugated dienes (CD) in the presence of 1.6 IxM CuSO4 and calculation o f the following parameters: lag time, rate of propagation, and maximal amount of conjugated dienes (maxCD). Results: In the LDL isolated from participants on the MUFA-rich diets, lag time was greater while the rate of propagation and maxCD were smaller than in LDL from subjects on the PUFA-rich diet, indicating that MUFArich diets increase the resistance of LDL to oxidation. Surprisingly, the olive and rapeseed oil diets, despite their much higher content of unsaturated fatty acids, also rendered the LDL less susceptible to oxidation compared to the SAFA-rich diet (Table). SAFA
Lag time (rain)
68±8 ° * . t
Rate o f propagation (nmol CD/min/mg LDL)
1.8 +0.2 ° ' , t t
2.1+0.4 " ' t t
MaxCD (nmol CD/mg LDL)
845=7° ° ' t ~'
*p < 0.05, " ' p < 0.001: all oils vs. SAFA; tp < 0.01. t t p < 0,001: olive or rapeseed oil vs. smailowcr oil
Conclusions: Compared to PUFA-rich diets, diets rich in MUFA protect LDL from oxidation. Moreover, LDL from subjects on MUFA-rich diets is less susceptible to oxidation even than that from persons consuming a diet rich in SAFA. A possible explanation is that the normally observed enrichment of LDL in linoleic acid can be reduced by a diet high in MUFA, because oleie acid may specifically compete with linoleic acid for incorporation into LDL during particle assembly.
HYPERTENSION TREATMENT WITH DOXAZOSIN SIGNIFICANTLY DECREASES FEMORAL WALL THICKNESS IN MALES WITH HYPERCHOLESTEROLEMIA AND PERIPHERAL ATHEROSCLEROTIC DISEASE N. Hoogerbrugg¢, E. de Groot, Th. Stijnen, M. de Ridder, L.H.M. de Heide, H. Jansen. Erasmus Medical Center Rotterdam, The Netherlands Observational studies suggest a synergistic effect o f hypertension and hyperiipidemia on the progression of atherosclerosis. Doxazosin (doxa) is an effective antihypertensive drug with favorable effects in plasma lipids. A randomized, blinded study was performed, to determine whether doxazosin (doxa) alters the risk for progressive atherosclerosis different from standard treatment with hydrochlorothiazide (HCTZ). Eighty male patients (59-1-7 years) with peripheral atherosclerosis and moderately increased cholesterol levels (6.1+0.8 mmol/l) were treated for hypertension, either with doxa (n = 39) or HCTZ (n = 41). The primary outcomes were the effects on wall thickness (IMT) of the femoral arteries and fasting lipid levels after 3 years of treatment. Results: Doxa and HCTZ were equally effective in normalizing the blood pressure. After 3 years of treatment with doxa, triglyceride concentration was decreased by 23% (p < 0.01) and HDLc was increased by 20% (p < 0.01). In contrast, treatment with HCTZ did not affect plasma lipid levels. Treatment with doxa significantly decreased the mean as well as the maximal IMT o f the femoral arteries, by 13 and 15% respectively (p < 0.01). Treatment with HCTZ did not affect IMT of the femoral arteries. Conclusions: Hypertension treatment with doxazosin improves dyslipidemia, moreover it reduces femoral arterial wall thickness in males with
71st EAS Congress and Satellite Symposia