Organochlorine pesticides residues and heavy metals in soil and vegetables

Organochlorine pesticides residues and heavy metals in soil and vegetables

Abstracts / Toxicology Letters 211S (2012) S43–S216 icitrin did not increase with time and was only half of that of syringetin and myricetin-3 ,4 ,...

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Abstracts / Toxicology Letters 211S (2012) S43–S216

icitrin did not increase with time and was only half of that of syringetin and myricetin-3 ,4 ,5 -trimethylether. Interestingly, no metabolites of these flavonoids could be detected which might at least in part be due to their low intracellular amounts. Absorption as well as intracellular distribution was also evidenced by using the fluorescent dye “Naturstoff reagent A” (2-aminoethyldiphenylborinate; NSRA). Fluorescence microscopy indicated a predominant cytosolic distribution of the employed flavonoids. In C. elegans, the flavonoids were exclusively distributed in the intestine as visualized by NSRA. The antioxidative capacity (TEAC and DCF assay) decreased with increasing methyl groups in the B-ring. Myricetin-3 ,4 ,5 -trimethylether (three methyl groups) was the least effective antioxidant in both assays compared to myricetin (no methyl groups). In conclusion, for exerting their biological effects, uptake and distribution of flavonoids in certain organs such as liver and gut is important and more research in that field is warranted. doi:10.1016/j.toxlet.2012.03.754

P34-13 Organochlorine pesticides residues and heavy metals in soil and vegetables Carmen Hura 1 , Bogdan Andrei Hura 2 , Cristina Perju 2 , Vasile Stoleru 3 INPH/Center Regional Public Health Iasi, Romania, 2 INPH/Centre Regional of Public Health, Romania, 3 University of Agricultural Sciences, Romania

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In this paper are presented the research results obtained in 2010/2011, regarding the assessment of some organochlorine pesticide residues and the assessment of the heavy metals (lead, cadmium and copper) from 80 soils samples and 25 vegetables samples from different growing systems (ecological and conventional). Methods: Determination of the organochlorine pesticide residues in soil and vegetables samples were performed by Gas chromatograph (GC Shimadzu, model 2100), equipped with an electron capture detector. Determination of the heavy metals in soil and vegetables samples was performed by atomic absorption spectrometry (AAS)-Schimadzu 6300, graphite furnace and autosampler. Results and conclusion: 1. In soil samples harvest from conventional ferm on Roman Farm and Tg.Frumos Farm were detected Endosulfan I (range 0.002–0.015 mg/kg); Endrin aldehide (range 0.004–0.01 mg/kg) and Endosulfan sulfate (0.001 mg/kg). Lead and cadmium concentrations ranged from 4.51 to 6.58 mg/kg and from 0.14 to 0.4 mg/kg, respectively. Cooper concentrations ranged from 20.73 to 31.59 mg/kg. 2. In vegetable samples from conventional farm, the content of organochlorine pesticide residues in some samples analysed were none detectable. In others samples, tomatoes, papper were detected heptachlor epoxid residues (range 0.001–0.006 mg/kg); endosulfan I residues (range 0.001–0.003 mg/kg) and endrin aldehide residues, but in admissible limits (<0.01 mg/kg).

from 0.0 mg/kg (tomatoes) to 0.60 mg/kg (eggplant). The cadmium concentrations not detected in no vegetable samples. doi:10.1016/j.toxlet.2012.03.755

P34-14 On chemo-toxicology: Pathophysiological reactions on toxicants Eva Neu 1 , Michael Ch. Michailov 1 , Viktor Foltin 2 , Walter Seidenbusch 3 , Joachim Milbradt 4 , Jakob Stiglmayr 4 , Janka Foltinova 2 1

Inst. Umweltmedizin c/o ICSD/IAS e.V., Germany, 2 Inst. Histology, Univ, Bratislava, Slovakia, 3 Inst. Exp.Physik, Univ. Innsbruck, Austria, 4 Bot. Garten, Univ. Erl.-Nürnberg, Germany

Introduction: The permanent increase of chemicals in environment (nutrition, etc.) needs indicators for functional disturbances on organ and cellular level: on example of toxic influence of effector and synaptic excitable systems are demonstrated immediate pathophysiological motor and electrical reactions in angio-cardial and urogenital preparations. Method: Motor and electrical activity of excitable tissue of fish (A), guinea-pig (B–D) [Ref.]. Results of recent and earlier evaluation of systematic investigations in the last three decades (2010–1980) concerning immediate motor and electrical reactions on toxicants are summarized. A. Cardio-vascular motor activity (salmo gaidneri): Alcohols (methanol, ethanol, butanol: 0.0001–0.5%), heavy metals (Cd, Hg, Mn: 0.01–100 ␮M), pyrethroids altered motor patterns (atrium/ventricle), also induced tonic contractions of aorta/bulbus. B. Urogenital motor activity: Xenobiotics - pyrethroids (cyper/deltamethrin), chlorophenols, herbicides (0.1–100 ␮M) inhibited, heavy metals (HgCl2 ) increased spontaneous phasic detrusor/pyeloureter contractions (1–5/min). C. Neuro-muscular regulation: These toxicants had inhibitory effects on neurogenic contractions to electrical stimulation (10/100 Hz, 0.3 ms, 3 s). D. Electrical activity: Intracellular recording of single detrusor myocytes: Cypermethrin and pentachlorophenol (1–10 ␮M) transformed electrical spike activity into a burst-plateau one, similar to stretch, i.e. change of mechano-sensitive ionic channels. Conclusion: An integrative toxicology, supporting UNOAgenda21 for better health, needs testing of toxicants on excitable systems to develop very sensitive indicators for the onset of alterations, leading e.g. to cardio-vascular (e.g. arrhythmia, hypertension) and urogenital (e.g. incontinence, overactive bladder, etc.) functional disturbances. References EUROTOX-2011-Paris Tox. Letters 205S, S203/S298. IUTOX2004-Tampere Tox. Appl. Pharmacol. 197/3:244/297; 2001Brisbane Toxicology 164/1–3:P1B25/P1C48; 1992-Rome Tox. Lett. Suppl., 159/160. Eur. Congr. Pharmac. Sci.-EUFEPS. 2000-Budapest Eur. J. Pharm. Sci. 11/S1:S94/S121–S122. 1998-Milan 6/S1:216/217 (see also Michailov/Neu et-al.). doi:10.1016/j.toxlet.2012.03.756

Lead concentrations ranged from 0.0 (tomatoes) to 4.35 mg/kg (cabbage); cooper concentrations ranged from 0.2 mg/kg (cucumber) to 0.80 mg/kg (eggplant); manganese concentrations ranged

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