--In vivo labeling of DNA probes with 5-BrdU. - - T h e sulfone system. --Colloidal gold as ~t marker in molecular biology: the use of ultra-small gold probes. --Direct peroxidase labeling of hybridization probes and chemiluminescence detection. - - A highly sensitive method for detecting peroxidase in in situ hybridization or immunohistochemical assays. - - T h e SNAP system. --Specialized nonradioactive detection systems --Overview of colorimetric, chemiluminometric and flurometric detection systems. --Colorimetric systems. --Luminescence syslEems. --Fluorescence syste.ms. --Enhanced systems --Overview of amplification systems. --Enhanced signal generation by target amplification. --Signal amplification system: substrate cascade. --Application formats --Overview of application formats. --Blot formats: nucleic acids. --Blot formats: proteins and glycoproteins. --In situ formats. --Quantitative formats. Each chapter contain,~ a short introduction, a detailed description of the method with laboratory protocols, troubleshooting tips and references and is accompanied by a list of the necessary standard reagents, buffers, and nonradioactive compounds. Two appendices compile the addresses of suppliers for the required reagents and equipment. The central aim of this book is to introduce the reader to the principles of the various methods and also to provide a collection of the respective protocols. The book covers the wide spectrum of general and specialized technology, mainly on the basis of laboratory protocols. It provides a useful, practical tool for nonradioactive bioanalysis, stimulating the direct and convenient use of nonradioactive labeling and detection methods in experiments in basic and applied fields of genetic engineering, biotechnology and medicine. All the bioanalytical labeling and detection techniques are gathered together in one source in this book, including: nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids (such as DIG, Biotin, BrdU, Sulfone, Immunogold, Silver Enhancement and SNAP), as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of nonisotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis, such as blot, colony/plaque and in situ hybridization formats, quantitative formats and also nonradioactive techniques for nucleic acid sequencing and amplification. This book would be useful for people working in the fields of endocrinology, biology, molecular biology physiology and biophysics, as well as for advanced students.
Osteoporosis: Etiology, Diagnosis, and M a n a g e m e n t - - S e c o n d Edition. Edited by B. L. Riggs and L. J. Melton III. Published 1995 by Lippincott-Raven, Philadelphia. ISBN: 0-7817-0275-5. Osteoporosis is one of the critical diseases facing the aging population and is one of the most important disorders encountered in clinical practice. Worldwide, fractures from osteoporosis occurring each year are projected to increase to 6.3 millio~Lin 2050 from 1.7 million in 1990. Many of the causes and risk factors for bone loss have been identified by physiologic and epidemiologic studies. A relationship between the immune system and cells of the osteoclast's lineage has been discovered. The bone cell remodeling cycle has been defined and its kinetics measured. Bone cells have been shown to respond not only to systemic factors, but to a myriad of local regulators and growth factors. Diagnostic methods have improved dramatically. Bone density at the site of fractures can now be measured more precisely and bone turnover can be assessed conveniently by measuring an ever-growing number of newly discovered circulating bone-related proteins. Despite belated understanding of the enormity of the problem of osteoporosis, prospects have never been better for bringing the disease under control. This second edition iprovides up-to-date knowledge in a number of basic and clinical disciplines related to osteoporosis.
The individual chapters of this book have been written by international experts in their respective areas, under five main topics, as follows:
--Basic science - - T h e cells of bone. --Biochemistry of bone. -- B o n e remodelling. --Biomechanics of fractures.
--Pathophysiology --Inadequate peak bone mass. --Estrogen deficiency. --Age-related factors. --Secondary osteoporosis.
--Clinical presentation --Clinical spectrum. --Epidemiology of fractures.
--Diagnosis --Radiological aspects. -- B o n e densitometry. - - B o n e histomorphometry. --Biochemical markers for the assessment of bone turnover.
--Management --Prevention. --Management of menopause. - - D r u g therapy: calcium and vitamin D. - - D r u g therapy: formation-stimulating regimens. --Musculoskeletal rehabilitation. --Quality of life. --Practical clinical management. This book would be useful for people working in pathophysiology, physiology, biochemistry, biology and molecular biology, as well as for advanced students.
The Polymerase Chain Reaction. Edited by K. B. Mullis, F. Ferre and R. Gibbs. Published 1994 by Birkhfiuser Verlag AG, Basel, Boston. 432 pp. ISBN (hardback): 3-7643-3607-2 and 0-8176-3607-2. Price: D M 168.00/£65.00. ISBN: 3-7643-3750-8 and (hardback) 0-8176-3750-8. Price: D M 98.00/£36.50. This is the first comprehensive handbook on polymerase chain reactions. Edited by the inventor of PCR and Nobel Prize winner in Chemistry for 1993, Kary Mullis and two prominent experts in the field, it provides the most up-to-date methodological protocols from the world's leading laboratories, as well as exciting new techniques and enhanced applications. PCR has to a surprising extent transformed the way molecular biology is done and has become an integral part of the DNA laboratory. In this book, the chapters covering applications provide not only protocols, but descriptions of how PCR has revolutionized the authors' various fields. Future enhancements of PCR, as well as new potential uses are also discussed. The main topics covered in this book are as follows:
--Methodology --Basic methodology. --Quantification. --Nonisotopic detection. --Instrumentation. --Sequencing.
--Applications --General applications. --Genetic analysis. --Assessment of therapy effectiveness. --Diagnostics.
--PCR and the world of business This book would be useful for people working in the fields of biochemistry and molecular biology, as well as for advanced students.