Recombinant DNA containing the herpes simplex gene, transformant animal cell cultures and production of herpes simplex virus protein, cloning for use in vaccine

Recombinant DNA containing the herpes simplex gene, transformant animal cell cultures and production of herpes simplex virus protein, cloning for use in vaccine

Patent Report gp120; (ii) the partial amino acid sequence extending between amino acids 53 and 122 of the gp41 protein; (iii) the partial amino acid...

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gp120; (ii) the partial amino acid sequence extending between amino acids 53 and 122 of the gp41 protein; (iii) the partial amino acid sequence extending between amino acids 123-163 of gp41; (iv) the partial amino acid sequence extending between amino acids 196-217 of gp41; and (v) the partial amino acid sequence extending between amino acids 30-181 of gpl20. Also claimed are recombinant D N A and plasmids coding for the polypeptides, and the HTLV-III isolate ~.HAT-3, a genomic clone of an HTLV-III prophage. (I) Is used for detection of antibodies against HTLV-III and as a vaccine. 012-88 Newcastle disease virus gene clones comprising polynucleotides encoding the HN and/or F protein of Newcastle disease virus RNA; vaccine

Nat. Res. Develop. Corp. Eur. 227 414; 1 July 1987 An artificial polynucleotide (I) encoding an HN and/or F polypeptide of Newcastle disease virus (NDV) DNA, a bioprecursor of the polypeptide, or an epitope of the polypeptide and an artificial nucleotide (II) complementary to (I) are claimed. The polynucleotides are useful for preparing a probe for extracting similar genes from a gene library or for identifying the presence of NDV virions in a sample obtained from poultry. They are also used for the production of polypeptides by recombinant D N A technology in an expression vector containing a strong promoter cloned in Escherichia coli. The eDNA is prepared by transcribing genomic R N A to yield an R N A : D N A hybrid, dC-tailing the hybrids, annealing them to dG-tailed plasmid vector and transforming a bacterial host. Clones are selected by antibiotic resistance and sensitivity and further verified by colony hybridization to probe separately prepared NDV eDNA. Appropriate portions of D N A inserts are ligated to obtain D N A complementary to the full length gene. The invention is useful in the diagnosis of NDV and for production of viral proteins for use in poultry vaccination. 013-88 Recombinant DNA containing the herpes simplex gene, transformant animal cell cultures and production of herpes simplex virus protein, cloning for use in vaccine

Ki~gaku-Oyobi-Kessei-Ryoho-Kenkyusho Jpn 2115 288; 26 May 1987 A process is described for cloning the herpes simplex virus (HSV) glycoprotein gene and comprises incorporating the gene into an Escherichia coli plasmid vector with a promoter region operable in animal cells. The HSV glycoprotein is a promising candidate for a HSV vaccine, but is normally difficult to purify. The gene encoding the glycoprotein is cloned in E. coli. To increase the efficiency of gene expression, the hydrophilic C-terminal of the gene is removed and the remaining gene is cloned into an expression vector composed of pBR322 and SV40 virus early promoter. The expression vector produced is used to transform animals cells (e.g. CHO T K - , CHO D H F R - ) and transformants are cultured to produce the HSV glycoprotein. 014-88

Production of polypeptides in virally infected insect host cells vector construction, application for e.g. hepatitis B virus surface antigen preparation for vaccine development

Microgenesys Eur. 228 036; 8 July 1987 The production of a selected polypeptide in an insect host cell comprises: (a) isolating a first D N A sequence, including a viral promoter from a virus capable of infecting the host cell; (b) isolating a second D N A sequence encoding the desired polypeptide; (c) combining the first and second D N A sequences to form a continuous third strand in which the 64 Vaccine, Vol. 6, February 1988

second sequence lies adjacent to and in-frame with the promoter, and which contains transcription termination signals; (d) infection of the host cells; and (e) growth of the cells and collection of the gene product. The polypeptide may be somatotropin; interferon; a polypeptide derived from the envelope of an AIDS associated retrovirus or from its nucleoprotein core; Plasmodium sp. polypeptide; an Epstein-Barr virus polypeptide; an immunoglobulin, or especially hepatitis B virus surface antigen. The genes expressed in this way may give improved yields or may have folding so that the viral epitope is presented better for vaccine effectiveness. Suitable insect hosts are Sporodoptera frugiperda, Trichoplusia ni, Heliothis zea or Manduca sexta. 015-88 New DNA molecules coding for Plasmodium falciparum blood stage antigens expressible in transformed microorganisms to give products useful as immunogens in vaccine

Walter & Eliza-Hall-Inst. Med. Res. World 8703 882; 2 July 1987 New D N A molecules (I) code for at least part of one of the following Plasmodium falciparum antigens: rhoptry protein; acidic basic repeat antigen (ABRA), or the antigens of clones Ag169, Ag303, Ag358, Ag361, Ag372, Ag394 or Ag501, or other cross-reactive antigens. Also new are (1) recombinant molecules, and cloning vectors containing (I) linked to an expression control sequence, and (2) host cells containing these recombinant molecules, etc. eDNA was isolated from strain FCQ27/PNG and inserted into vector phage X-gt11Amp3. A clone (Ag44) was obtained that encoded part of the rhoptry protein. A B R A was located in mature schizonts. The DNA, and corresponding amino acid, sequences of both the rhoptry protein and A B R A are given. Other clones were identified using antibodies rasied against P. falciparum antigens expressed in Escherichia coli using the ~.-Amp3 vector. The mol.wt, D N A sequence and stage specificities for some of these clones are also reported. The antigenic polypeptide products are useful for stimulating the immune response of humans against P. falciparum. 016-88 New polypeptides for protecting chickens against coccidiosis, vaccines obtained by recombinant DNA techniques

Solvay Ausl. 8665 869; 4 June 1987 D N A molecules encoding antigenic proteins are described, together with eDNA and m R N A molecules which encode them. The proteins are capable of inducing in a chicken an immune response conferring protection against infection by Eimeria necatrix, Eimeria tenella or Eimeria maxima. The proteins have been characterized with respect to mol.wt and amino acid sequence. A recombinant cloning vector is new which contains the cDNA or m R N A sequence encoding the antigenic polypeptide of interest. The vector is used for expression of the protein in bacterial cells, e.g. those of Escherichia coli, following transformation. Monoclonal antibodies have also been produced which may be used for antigenic polypeptide purification etc. D N A is isolated from Eimeria oocysts and cloned. The recombinant products may comprise fusion proteins produced by E. coli, and these are used for the production of a vaccine against coccidiosis. 017-88 Pseudorabies virus vaccine comprising monocional antibody which can be mass produced by hybridoma culture and has high specificity

Upjohn World 8704 075; 16 July 1987 A method of treating pseudorabies virus (PRV) infection in swine comprises administering a monoclonal antibody (MAb)