The Biology and Biochemistry of Pulmonary Alveolar Epithelial Cells

The Biology and Biochemistry of Pulmonary Alveolar Epithelial Cells

Appendix 9 The Biology and Biochemistry of Pulmonary Alveolar Epithelial Cells TABLE 1 Dependence of Phenotype on Substrata Substrata Shape Disatu...

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Appendix 9

The Biology and Biochemistry of Pulmonary Alveolar Epithelial Cells TABLE 1 Dependence of Phenotype on Substrata

Substrata

Shape

Disaturated Phosphatidylcholine Synthesis

Tissue culture plastic

Spread

Decreased

Decreased

Bovine corneal extracellular matrix

Cuboidal

Decreased

Decreased

Human lung matrix

Spread

Decreased

Decreased

Amniotic membrane Interstitial side Basement membrane side

Spread Cuboidal

Decreased Maintained

Decreased Decreased

Engelbreth-Holm-Swarm matrix

Cuboidal

Maintained

Decreased

Floating collagen membranes

Cuboidal

Decreased

Decreased

Feeder cell layers

Cuboidal

Maintained

Decreased

Phosphatidylglycerol Synthesis

787

788 Appendix 9

TABLE 2 Secretory Products of Alveolar Epithelial Cells Surfactant lipids and proteins Complement components Prostaglandins Lysozyme Glutathione (?) GSH is a diffusable product of the airway lining cells. However, it is not known if GSH is actively secreted from epithelial cells. GSH is highly unlikely to be taken back up into cells.

TABLE 3 Epithelial Cell Enzymes Alkaline phosphatase Acid phosphatase Aryl sulfatase b-N-acetylglucosaminidase Vitamin K-dependent carboxylase Protein kinase C Cyclic AMP-dependent protein kinase Cytochrome P450 dependent monooxygenase NADPH dependent cytochrome P450 reductase