Biosensors & Bioelectronics Vol. 11 No. 1/2 (1996)
C.E. Hall & E.A.H. Hall of the University of Cambridge report on ‘Tuning of glucose oxidase modified alkylacrylate polymers employed in amperometric sensor systems’. The efficiency of copolymers of methyl and glycidyl methacrylates as supports for the immobilisation of glucose oxidase is discussed in terms of polymer molecular weight, polymer composition and reproducibility. Copolymer molecular weight could be correlated with the inverse of the signal obtained in response to glucose, from glucose oxidase modified polymer coated electrodes. The magnitude of the response to glucose was also dependent upon the composition of the polymer. Contact: University of Cambridge. Institute Tennis Court Road. of Biotechnology, Cambridge, UK.
on ‘Novelsensing system for glucose based on the complex formation between phenylborate and fluorescent diol compounds’. This study was performed to demonstrate the feasibility of a novel glucose sensing system in an aqueous milieu based on a change in the fluorescence on competitive binding between a fluorescent diol compound and glucose toward a phenylboratecompound. The relationship between the glucose concentration and fluorescence intensity was linear in the range of 0 to 500 mg/dl of glucose. These functionalities could be incorporated on the tip of an optical fiber to construct an opto-sensing system for glucose. Contact: Department of Materials Science, Research Institute for Biosciences, Science University of Tokyo, 2641 Yamazaki, Noda, Chiba 2 78. Japan.
UK - Simultaneous determination of follicle stimulating hormone and luteinising hormone In ANAL. CHIM. ACTA (3 10/2 (25 l-256) 1995) D.J. Pritchard, H. Morgan & J.M. Cooper of the University of Glasgow report on ‘Simultaneous determination of follicle stimulating hormone and luteinising hormone using a multianalyte immunosensor’. The authors describe a technique for constructing a multianalyte immunosensor for the quantitative determination of the human gonadotrophin hormones follicle stimulating hormone (FSH) and luteinising hormone (LH). The assay is a based upon the electrochemical detection of two horseradish peroxidase labelled antibodies using a ferrocene mediated system. Results showed a good correlation with an established clinical diagnostic technique based dissociation-enhanced lanthanide upon fluorometric immunoassay. Contact: Department of Electronics. Electrical Engineering, University of Glasgow, Glasgow G12 8QQ, UK.
Poland - Evaluation of potential glucose biosensor components In BIOMETALS 8/3 (257-262) 1995) K. Gasiorowski, K. Szyba, J. Urban, M. Cieslak-Golonka,S.M. Zakeeruddin, M. Gratzel & D.M. Fraser of the Department of Basic Medical Sciences, Medical Academy report on ‘Mutagenic activity of group VIII metal-organic complexes in the Ames test: Evaluation of potential glucose biosensor components’. novel trisFive (4,4’-substituted2,2’-bipyridine) complexes of the group VIII metals [Fe(II), Ru(II) and Os(II)] were assayed in the Ames test to evaluate their mutagenic activity. For the bipyridinecomplexes, some complexes did exhibit mutagenic activity in the circumstances of this test, whereas others did not. There was no specific correlation with the identities of the metal and ligand type; the possible explanations of the results are discussed in this paper. In view of our mutagenic test results, such complexes found to be mutagenic should not be taken into consideration as components of implanted glucose sensors in future in vivo experiments. Contact: Department of Basic Medical Sciences,Medical Academy, 14 Kochanowski Str, 51-601 Wroclaw, Poland.
Japan - Novel sensing system for glucose In J. BIOCHEM. (117/6 (1145-l 147) 1995) K. Kataoka, I. Hisamitsu, N. Sayama, T. Okano & Y. Sakurai of the Science University of Tokyo report